CHAPTER 12

Surface and Equipment Asepsis

LEARNING OBJECTIVES

After completing this chapter, the student should be able to do the following:

image Determine which operatory surfaces may be involved in the patient-to-patient spread of microbes.

image Differentiate between clinical contact surfaces and housekeeping surfaces.

image List the operatory surfaces that should be covered with barriers before patient care.

image Describe how to place and remove surface covers properly.

image List the types of surface disinfectants and describe their properties.

image Differentiate between low-, intermediate- and high-level disinfectants and give examples when each should be used.

image Read with understanding the labels on disinfectants.

image Describe the importance of precleaning before surface disinfection.

image Describe how to preclean and disinfect contaminated surfaces and equipment.

image Describe how aseptically to retrieve and distribute clinical supply items.

KEY TERMS

Antibiotics

Antiseptics

Bactericidal

Bioburden

Chlorine

Clinical Content Surfaces

Disinfectants

Disinfection

Fungicidal

High-level Disinfectant

Hospital Disinfectant

Housekeeping Surfaces

Intermediate-level Disinfectant

Iodophors

Low-level Disinfectant

Phenolics

Precleaning

Quaternary Ammonium Compounds

Sodium Hypochlorite

Sporicidal

Spray-wipe-spray

Sterilants

Surface Covers

Tuberculocidal

Virucidal

Wipe-discard-wipe

TYPES OF ENVIRONMENTAL SURFACES

Two types of dental environmental surfaces are related to disease spread: clinical contact surfaces and housekeeping surfaces. Clinical contact surfaces are surfaces that may be touched frequently with gloved hands during patient care or that may become contaminated with blood, saliva, or other potentially infectious material and subsequently contact instruments, devices, hands, or gloves. Box 12-1 gives examples of such surfaces. Housekeeping surfaces are surfaces that do not come into contact with hands or devices used in dental procedures (e.g., floors, walls, and sinks). The clinical contact surfaces need to be treated properly before they become involved in the care of the next patient. Housekeeping surfaces can be treated at the end of the day.

BOX 12-1   EXAMPLES OF SURFACES SUSCEPTIBLE TO CONTAMINATION DURING PATIENT CARE ACTIVITIES

Headrest on chair

Chair control buttons

Light handles

Light switch

Bracket table

Handpiece control switches

Handpiece hoses

Evacuator hoses

Evacuator control

Air/water syringe handle

Air/water syringe hoses

Drawer handles

Countertops

X-ray unit handle and cone

X-ray unit controls

X-ray view box switch

Dental team chair backs

Supply containers and bottles

Light curing handle and tip

Mirror handles

Faucet handles

Shade guides


Usually more easily covered than precleaned and disinfected, although all surfaces lend themselves to covering.

Different microorganisms may survive on environmental surfaces for different periods, as determined in laboratory studies. For example, Mycobacterium tuberculosis may survive for weeks, whereas the herpes simplex virus dies in a matter of seconds to minutes. Various conditions influence the survival time of microorganisms in the environment, including humidity, temperature, the presence of nutrients and blood or saliva, and the general surface properties of the microorganism. Accurately predicting how long any microorganism actually may survive on a dental office surface is impossible because of these unknown variables. Thus if a surface becomes contaminated with saliva, blood, or other potentially infectious material, the safest approach is to assume that it indeed contains live microorganisms that must be removed or killed before the surface is involved in the treatment of the next patient.

Two general approaches to surface asepsis are these: one is to prevent the surface or item from becoming contaminated by use of a surface cover, and the other is to preclean and disinfect the surface after contamination and before reuse. Each approach has advantages and disadvantages, and usually an office will use a combination of both approaches (Table 12-1).

TABLE 12-1

Surface Covers Versus Precleaning and Disinfection

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SURFACE COVERS

The best way to manage surface asepsis from an infection control point of view is to prevent contamination of the surface so that it will not have to be precleaned and disinfected before reuse.

Types of Surface Covers

Contamination can be prevented by proper placement of a surface cover before an opportunity for contamination exists. Surface covers should be impervious to fluids to keep microorganisms in saliva, blood, or other liquids from soaking through to contact the surface. Examples of appropriate material for surface covers include clear plastic wrap, bags, or tubes, and plastic-backed paper. Some plastics are designed specifically for use as surface covers in the office in that they have the shape of the item to be covered (e.g., air/water syringe handle covers, hose covers, and pen covers). Some sheets of plastic also have a slightly sticky substance on one side to hold them on the surface. Other plastics (e.g., some food wraps) have a natural clinging ability on contact with a smooth surface. Some plastic bags are available with drawstrings that hold the bag around an item to be protected. To reduce costs, one can use thin rather than thick plastic sheets or bags as long as they are not punctured by the surface being covered. Patient bibs are made of plastic-backed paper and also can be used to cover flat operatory surfaces, although thin plastic sheets may be less expensive.

Use of Surface Covers

Dental units come in several shapes and sizes with different positioning of the handpiece control system and light and other accessories. Thus the sizes and shapes of surfaces to be covered vary from one office to the next, but general procedures for use of surface covers are the same (Procedure 12-1).

PROCEDURE 12-1

Use of Surface Covers

GOAL: To prevent contamination of surfaces

Materials Needed

Appropriate surface covers

Gloves

Cleaner/disinfectant (when used will need gloves, mask, clothing, and eye protection)

Material safety data sheet for cleaner/disinfectant

1. Apply appropriate surface covers before the surfaces have a chance to become contaminated with patient material.

Rationale: Applying the covers after the surfaces become contaminated is a waste of money and time because the contaminated surfaces will have to be precleaned and disinfected anyway when the patient leaves. The covers also may become contaminated themselves when they are applied to a contaminated surface and could contribute to cross-contamination of the next patient.

2. If the surfaces to be covered have been contaminated previously with patient materials, preclean and disinfect the surface and then remove gloves and wash hands before applying the surface covers.

Rationale: Covers need to be placed on cleaned and disinfected surfaces as explained in step 1. Because the surfaces and the covers are not contaminated, one may use bare hands to place the covers.

3. Place each surface cover so that it protects the entire surface and will not come off when the surface is touched.

Rationale: Not covering the entire surface defeats the purpose of using surface covers: to prevent contamination. Also, if the covers come off before the end of the appointment, the surfaces will become contaminated.

4. Wear gloves during removal of surface covers after patient care or other activities are completed.

Rationale: The surface covers are contaminated with patient materials.

5. Carefully remove each cover without touching the underlying surface.

Rationale: This step prevents the underlying surface from becoming contaminated. Covering surfaces is intended to replace precleaning and disinfecting between patients. If the previously covered surface becomes contaminated, then one must perform extra work.

6. If a surface is touched during removal of the cover, preclean and disinfect the surface.

Rationale: Covered surfaces must be free of contamination as explained in step 1.

7. Discard used covers into the regular trash unless local laws consider these items as regulated waste; then dispose of them as indicated by the law.

Rationale: Federal law (Occupational Safety and Health Administration) states that solid nonsharp waste must be dripping wet or caked with body fluid or partially dried body fluid for it to be handled as regulated waste. Surface covers are never caked or saturated with body fluids unless a blood or saliva spill occurs.

8. Remove and discard contaminated gloves, wash hands, and apply fresh surface covers for care of the next patient.

Rationale: The gloves become contaminated from contact with the used surface covers. Washing hands after removal of gloves is standard procedure.


In most states, nonsharp contaminated waste such as surface covers is not considered as regulated waste unless an item is soaked or caked with blood or saliva that would be released if the item is compressed.

Clear plastic bags are available in various sizes and are easy to use. For example, if a chair has control buttons on the side, one can use one bag to cover the headrest and the buttons (Figure 12-1). Wraparound backs of side chairs used by the dental team may be touched during patient care and can be covered with a large plastic bag (Figure 12-2). On units with a bracket table, one may use one bag to cover the handpiece control unit and the bracket table (Figure 12-3 and Figure 12-4). One can cover hoses and connectors with plastic tubing that is secured with tape or a rubber band at the connector or can force a narrow, long plastic bag over the connector end (Figure 12-5).

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FIGURE 12-1 Surface cover for headrest and chair buttons.

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FIGURE 12-2 Surface covers for backs of chairs used by the dental team.

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FIGURE 12-3 Surface cover for a control unit and instrument tray holder.

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FIGURE 12-4 Surface covers for separate control unit and instrument tray holder.

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FIGURE 12-5 Surface covers for hoses and connectors. A, Saliva ejector connector, air/water syringe handle, and high volume evacuation connector. B, Various connectors on holders.

Light handles and light switches commonly are touched during patient care and can be covered with plastic wrap or bags, depending on their shape (Figure 12-6). Some lights have removable handles that can be cleaned and heat sterilized before reuse. Covering the air/water syringe handle with plastic wrap to prevent contamination is better than trying to preclean and disinfect properly around the buttons that tend to retain debris (see Figure 12-5). One can cover the heads of x-ray units and the control panels (Figure 12-7), and if the water at sinks is not controlled by elbow levers, foot pedals, or automatic devices, one can cover faucet handles with plastic bags (Figure 12-8).

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FIGURE 12-6 Surface covers for a light handle and switch.

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FIGURE 12-7 Surface covers for an x-ray machine.

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FIGURE 12-8 Surface covers for a sink faucet.

PRECLEANING AND DISINFECTION

Precleaning and disinfection best lend themselves to nonelectric surfaces that are smooth and easily accessible for facilitating good contact with the decontaminating chemicals. Procedure 12-2 describes general procedures for precleaning and disinfecting surfaces.

PROCEDURE 12-2

Precleaning and Disinfecting Surfaces

GOAL: To clean and disinfect contaminated surfaces and equipment

Materials Needed

Liquid cleaner/disinfectant or disinfectant towelettes

Paper towels or gauze pads

Material safety data sheet for cleaner/disinfectant

Gloves, mask, clothing, and eye protection

1. Put on utility gloves, mask, protective eyewear, and protective clothing.

Rationale: Protective gear prevents contact with contaminants and chemicals through touching or splashing.

2. Choose a cleaner/disinfectant that is compatible with the surfaces to be cleaned and disinfected. Many manufacturers of dental equipment have determined which surface disinfectants are most appropriate for their products (e.g., dental chairs and unit accessories) from a material compatibility point of view.

Rationale: One wants to cause the least damage possible to the surfaces being decontaminated.

3. Confirm that the precleaning/disinfecting product(s) have been prepared correctly (if diluted) and are fresh (if necessary). Read and follow the product label directions.

Rationale: Using the product as directed helps ensure that the product works effectively.

4. For spray-wipe-spray: spray the surface with the cleaning/disinfecting agent and vigorously wipe with paper towels. Holding paper towels behind appropriate surfaces during the procedure will reduce overspray. Alternatively, saturate a paper towel or gauze pad with the cleaning/disinfecting agent and vigorously wipe the surface. Use a brush for surfaces that do not become visibly clean from wiping and consider covering them with a plastic barrier in the future. If cleaning large areas or multiple surfaces or large spills, use several towels or pads for cleaning so as not to transfer contamination to other surfaces. Disinfect the precleaned surface by respraying the disinfecting agent over the entire surface using towels to reduce overspray (or apply with a saturated pad). Let the surface remain moist for the longest contact time indicated on the product label (usually 10 minutes). Vertical surfaces may dry more quickly.

5. For wipe-discard-wipe: obtain a disinfectant towelette from its container, close the container lid and vigorously wipe (clean) the surface. Discard the towelette and obtain a fresh towelette and wipe the surface again for disinfection. Discard the towelette and let the surface dry.

Rationale: Proper precleaning (predisinfection) is essential to reduce the level of bioburden so the disinfecting step will have the best chance to kill remaining microbes. Using a disinfectant/cleaner begins to kill microbes on the surface during the cleaning step and helps protect the person doing the cleaning. Although the precleaning step removes/kills some microbes, the subsequent disinfection step helps ensure removal/death of the contaminates.

6. If the surface is still wet when ready for patient care, wipe dry. If the surface will come into direct contact with the patient’s skin or mouth, rinse/wipe off residual disinfectant with water.

Rationale: This prevents the cleaning/disinfecting chemicals from contacting the patient and staining clothing or irritating the skin.

Approaches to Precleaning and Disinfection

Surfaces to be disinfected first must be precleaned. Precleaning reduces the number of contaminating microorganisms and the blood or saliva present (referred to as bioburden) and facilitates action of the disinfecting chemical. Precleaning is an important step that one must not slight. The organic material in blood and saliva insulates microorganisms from contact with a disinfecting chemical and also may inactivate a portion of the active chemical in the disinfectant.

During the precleaning process, one may use regular soap and water, but use of a surface cleaner/disinfectant that contains detergents is best for the precleaning step and the disinfecting step. Using a disinfectant/cleaner for the precleaning step starts the killing process early and reduces the chances of spreading the contamination to adjacent surfaces. This (along with wearing heavy utility gloves, a mask, protective eyewear, and protective clothing) provides a little more protection to the person cleaning the surface. Water-based disinfectant-detergents (e.g., some synthetic phenolics, iodophors, and quaternary ammonium compounds—alcohols) solubilize organic materials such as blood and saliva and facilitate their removal. One also can use these same products in the disinfection step.

There are two approaches to precleaning and disinfection (see Procedure 12-2). One (using a liquid disinfectant/cleaner) is spray-wipe-spray and the other (using a disinfectant towelette) is wipe-discard-wipe. For spray-wipe-spray (Figure 12-9) spray the cleaner/disinfectant on the surface and wipe (preclean) the surface vigorously with a paper towel or gauze pad. Then respray the disinfectant/cleaner on the precleaned surface and let it remain moist for the longest contact time indicated on the disinfectant label (usually 10 minutes). If moisture remains, one should wipe it away with a paper towel. Some disinfectants are promoted as “one-step” products (i.e., spray and wipe). Although it is usually best to follow manufacturer’s directions on all products, it won’t hurt to use the one-step products in the two-step procedures (spray-wipe-spray). The two-step procedure becomes even more important should the postcleaning disinfecting contact time stated on the product label be shortened for some reason. If this is the case, at least the precleaning step will have removed/killed some of the microbes in the bioburden on the surface.

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FIGURE 12-9 Surface precleaning and disinfecting by the spray-wipe-spray technique. Precleaning consists of A, spraying with a detergent-disinfectant and B, wiping the surface to clean it. Disinfection is C, reapplying the detergent-disinfectant followed by the appropriate contact time.

Do not store paper towels or gauze pads in a disinfectant and use as wipes to avoid spraying. Wet the towels or pads just before use. Paper towels may be held behind some surfaces to catch overspray, if desired.

An alternative to spray-wipe-spray is the wipe-discard-wipe procedure using disinfectant towelettes. Pull a towelette from its container and wipe (preclean) the surface. Then discard that towelette. Obtain another towelette and wipe (disinfect) the surface, let the surface remain wet for the prescribed time, and discard the towelette. Dry off the surface if necessary. When using containers of disinfectant towelettes, keep the lid closed when not in use to prevent evaporation of the alcohol. Also periodically invert the container to redistribute any settled liquid.

If it is difficult to remove all of the bioburden from a given surface, then consider protecting it with a surface cover during future use. This is usually better than attempting to brush it clean and risk spreading contaminations to nearby surfaces.

Unfortunately, one cannot determine the effectiveness of disinfecting surfaces. Thus performing the procedures carefully and using the disinfectant as described on the product label is important.

Characteristics of Disinfectants

There are four general types of antimicrobial chemicals.

• Antibiotics (for killing microorganisms in or on the body)

• Antiseptics (for killing microorganisms on the skin or other body surfaces)

• Disinfectants (for killing microorganisms on environmental/inanimate surfaces or objects)

• Sterilants (for killing all microorganisms on inanimate objects)

The Centers for Disease Control and Prevention has categorized disinfectants based on their microbial spectrum of activity. This categorization follows and is described further in Table 12-2:

TABLE 12-2

Categories of Disinfecting/Sterilizing Chemicals

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EPA, Environmental Protection Agency.

Some, but not all, of these products can serve as high-level disinfectants and sterilants depending on the immersion time used.

A hospital disinfectant is one that has been shown to kill Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella choleraesuis.

The Centers for Disease Control and Prevention indicates that low-level disinfectants can be used on clinical contact surfaces if the product has a label claim of killing human immunodeficiency virus and hepatitis B virus in addition to being an EPA-registered hospital disinfectant.

Adapted from Centers for Disease Control and Prevention: Guideline for infection control in dental health-care settings, 2003, MMWR 52 (No. RR-17):66, 2003.

• Sterilant/high-level disinfectant (for killing all microorganisms on submerged, inanimate, heat sensitive objects)

• Intermediate-level disinfectant (for killing vegetative bacteria, most fungi, viruses, and Mycobacterium tuberculosis var bovis)

• Low-level disinfectant (for killing most vegetative bacteria, some fungi, and some viruses)

Labels on antimicrobial products can be confusing, but reading these labels carefully before using the product is very important. These labels commonly include the type of antimicrobial agent (active ingredients) and general properties such as the following:

• Virucidal (kills at least some viruses)

• Bactericidal (kills at least some bacteria)

• Fungicidal (kills at least some fungi)

• Tuberculocidal (kills the Mycobacterium tuberculosis var bovis bacterium)

• Sporicidal (kills bacterial spores, which means it is a sterilant)

• Hospital disinfectant (shown to kill the three representative bacteria: Staphylococcus aureus, Salmonella choleraesuis, and Pseudomonas aeruginosa)

The labels also commonly include the following:

• Specific microorganisms (genus and species) shown to be killed in laboratory testing (along with the necessary contact time)

• Directions for use of the product, including the need for precleaning

• Precautionary statements on handling the product

• Warnings such as toxic, poisonous, or flammable

• Treatment for accidental contact

• Storage and disposal information

• Shelf-life and use-life

• How to activate or dilute the product

• Name and address of the manufacturer/distributor, volume of the container, and Environmental Protection Agency (EPA) registration number

No perfect disinfectant exists that will kill all types of disease-producing microorganisms rapidly, have no toxic properties, be unaffected by organic materials, be odorless, not cause any damage to any surface, and be economical. Nevertheless, several classes of disinfecting chemicals have gained wide use in dentistry and medicine. These classes are identified by the type of antimicrobial agents present in the product, which are listed on the product label as active ingredients (Table 12-3). Because M. tuberculosis var bovis is more difficult to kill than most other microorganisms, disinfectants with tuberculocidal activity are considered as strong disinfectants (see Chapter 11, Figure 11-1, Mycobacterium species). Stronger disinfectants are active against nonenveloped hydrophilic viruses also, as described in Table 12-4. Use of a water-based disinfectant is reported to provide better cleaning of biologic material such as blood than use of an alcohol-based disinfectant. For dental infection control, a water-based surface disinfectant that is EPA-registered and tuberculocidal (such as iodophors, phenolics, or chlorines) is appropriate if used as directed by the manufacturer and careful precleaning is performed.

TABLE 12-3

Active Ingredients in Surface Disinfectants

Type of Disinfectant Example of Active Ingredient(s) Listed on Product Label
Chlorines Sodium hypochlorite
  Chlorine dioxide
Iodophors Butoxypolypropoxypolyethoxyethanol-iodine complex
Water-based phenolics  
Triphenolics o-Phenylphenol, o-benzyl-p-chlorophenol, and tertiary amylphenol
Dual phenolics o-Phenylphenol and o-benzyl-p-chlorophenol
PCMX p-Chloro-m-xylenol
Alcohol-based phenolics Ethyl or isopropyl alcohol plus
  o-phenylphenol or o-phenylphenol and tertiary amylphenol
  Isopropyl alcohol
Alcohols  
Quaternary ammonium compounds  
First generation Benzalkonium chloride
Second generation Alkyldimethylethylbenzyl ammonium chloride or alkyl- dimethyl-3,4-dichloro-benzyl ammonium chloride
Third generation Combination of first and second generation
Fourth generation Dioctyldemethyl ammonium bromide or didecyldemethyl ammonium bromide
Fifth generation Combination of first and fourth generation
Alcohol–quaternary ammonium compound A dimethyl-benzyl ammonium chloride plus isopropyl alcohol

Tuberculocidal.

Not tuberculocidal.

TABLE 12-4

Types of Viruses Used in Testing Disinfectant

Class Virus Solubility
Nonenveloped viruses Poliovirus type 1 Hydrophilic
  Coxsackievirus B1, B2 Hydrophilic
  ECHO type 6 Hydrophilic
  Rhinovirus type 17 Hydrophilic
  Adenovirus type 2 or 7 Intermediate
  Reovirus Intermediate
  Rotavirus Intermediate
  V-40 virus Intermediate
Enveloped viruses Herpes simplex 1 Lipophilic
  Influenza A2 Lipophilic
  Vaccinia Lipophilic
  Human immunodeficiency virus Lipophilic

Hydrophilic viruses do not have an envelope, and this makes them more difficult to kill with disinfecting chemicals and makes them more soluble in water.

Intermediate viruses do not have an envelope, but their susceptibility to killing by disinfecting chemicals is intermediate between lipophilic and hydrophilic viruses, and they are more soluble in lipid materials than other nonenveloped viruses.

Lipophilic viruses have a lipid envelope that makes them easier to inactivate with disinfecting chemicals and makes them more soluble in lipid materials than in water.

Manufacturers of disinfectants must submit testing data on the antimicrobial activity and safety of their products to the EPA. If the data are consistent with claims stated on the product labeling and other requirements are met, the EPA registers the product. The U.S. Food and Drug Administration also must grant marketing clearance to liquid chemical sterilants/high-level disinfectants labeled for use on medical devices such as heat-sensitive dental items.

CHLORINE COMPOUNDS

Chlorine compounds have been used for many years to disinfect drinking water, swimming pool water, and various inanimate surfaces. These agents are intermediate-level disinfectants, kill a wide variety of microorganisms, and are tuberculocidal. Sodium hypochlorite (which is the main chemical in bleach) is an example of a chlorine compound used to disinfect surfaces. One should note that commercial bleach (which contains about 5.25% sodium hypochlorite) is a good surface disinfectant at a 1:10 to 1:100 dilution with water, even though the bleach product is not an EPA-registered disinfectant. However, EPA-registered disinfectants containing sodium hypochlorite or other chlorine compounds are available. Sodium hypochlorite can damage fabrics and metal surfaces (particularly aluminum), and its activity is reduced in the presence of organic material. If one uses diluted commercial bleach as a disinfectant, one should prepare the solution fresh daily. One should wear gloves, protective eyewear, a mask, and protective clothing when using any disinfectant.

IODOPHORS

Iodine and iodine-alcohol mixtures (known as tinctures of iodine) are well-known killing agents but have some undesirable properties of corrosiveness, staining, irritation of tissues, and allergenicity. When iodine is complexed with certain organic materials, the compound is referred to as an iodophor (see Table 12-3). Most iodophors are intermediate-level disinfectants and retain the broad-spectrum antimicrobial activity (including tuberculocidal activity) of iodine, but they are less corrosive, are less irritating to tissues, and have reduced staining activity. Detergents are added to iodophor preparations used as surface disinfectants to enhance the cleaning ability of the solution. One may purchase iodophor disinfectants as concentrated solutions that must be diluted correctly before use. The diluted solution that is used should be prepared fresh daily, unless the manufacturer’s label indicates otherwise. Because hard water may reduce antimicrobial activity, iodophor disinfectants should be diluted with distilled or deionized water. Iodophors still may be slightly corrosive to some metals and may cause slight staining with repeated use on light-colored surfaces. However, one usually can remove this stain by wiping with alcohol. One should wear gloves, protective eyewear, a mask, and protective clothing when using any disinfectant.

ALCOHOLS

Isopropyl alcohol (isopropanol, sometimes referred to as rubbing alcohol) and ethyl alcohol (ethanol) have been used as antiseptics and disinfectants for many years because they are relatively nonirritating to the skin, although alcohol does “sting” when placed on mucosa (e.g., eye, mouth, and nostrils). Although alcohols at 50% to 70% concentration rapidly kill many microorganisms and are tuberculocidal, they evaporate rapidly when sprayed or wiped on surfaces. To counteract this drawback, extenders that retard evaporation of the alcohol after it is placed on a surface have been added to some isopropanol preparations sold as surface disinfectants. Nevertheless, alcohol has other properties that make it less desirable than other agents as a disinfectant. These properties include a reduction in activity by organic material, corrosiveness, and destruction of some plastic surfaces. Alcohols also do not solubilize protein material in blood or saliva well and have been reported to be poor cleaners. Alcohols dry out the skin because they tend to dissolve fat and oil that serve as natural skin moisteners. The “waterless” alcohol-containing hand rubs also contain special skin moisteners to reduce these drying effects. Although other agents are more appropriate than alcohol as surface cleaners/disinfectants, alcohol is an important component of some disinfectant preparations (see Table 12-3).

SYNTHETIC PHENOLICS

Phenol (also known as carbolic acid) has the distinction of being the first widely recognized disinfectant used in hospitals. Lord Joseph Lister suggested the use of phenol as an antiseptic during surgical procedures and as an environmental surface disinfectant more than 100 years ago. Although phenol clearly reduced postoperative infections, it was toxic to tissues, and its use on human beings was stopped. Several phenol-related (phenolic) compounds have been synthesized since and used for microbial killing. Thus these compounds are referred to as synthetic phenolics, with some use today as active ingredients in surface disinfectants (see Table 12-3) and in mouth rinses and handwashing agents.

Most of the synthetic phenolic disinfectants are intermediate-level tuberculocidal agents and may contain one, two (dual phenolics), or three phenolics (tirphenolics), as well as detergents to facilitate cleaning. Some of these disinfectants also contain alcohol. Some preparations must be diluted before use and should be prepared fresh daily unless otherwise stated on the product label. Some preparations are packaged in aerosol cans, and others are contained in pump-spray bottles. Some preparations may leave a film on the disinfected surface, degrade plastic surfaces with prolonged contact, or etch glass surfaces. One should wear gloves, protective eyewear, a mask, and protective clothing when using any disinfectant.

QUATERNARY AMMONIUM COMPOUNDS

ALCOHOL-FREE QUATERNARY AMMONIUM COMPOUNDS: Quaternary ammonium compounds are also called “quats.” These compounds are cationic detergents categorized as low-level disinfectants. A wide variety of quats has been developed over the years, and each time a new type of quat is developed, it is referred to as the next generation (see Table 12-3). All of the alcohol-free quat disinfectants have a low level of antimicrobial activity, and none are tuberculocidal. These disinfectants also may be inactivated by organic materials and soaps. Although they may be appropriate for disinfection of floors and walls, they are less desirable for items more directly involved in patient treatment that may be contaminated with body fluids. Because of these drawbacks, the American Dental Association recommended in 1978 that quaternary ammonium compounds (free of alcohol) not be used in dentistry. However, the Centers for Disease Control and Prevention has indicated that low-level disinfectants such as alcohol-free quats may be used on clinical contact surfaces if the product also has claims for killing human immunodeficiency virus and hepatitis B virus.

QUATERNARY AMMONIUM COMPOUNDS WITH ALCOHOL: The addition of alcohol to quaternary ammonium compounds enhances their antimicrobial activity. These intermediate-level disinfectants are tuberculocidal and are appropriate for use in dentistry.

EQUIPMENT DECONTAMINATION

General Considerations

Some basic considerations for equipment decontamination exist. In general, one should try to prevent the equipment from becoming contaminated in the first place. If this is not possible, one should select heat sterilization first. If the equipment cannot be heat sterilized, then one should use a liquid sterilant or an appropriate disinfectant for decontamination. Following the manufacturer’s directions for preventing contamination or for cleaning, sterilization or disinfection of dental equipment is important.

One should use the following principles to reduce the spread of microbes from dental equipment used in the mouth that will penetrate soft tissue or tooth structure (Figure 12-10).

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FIGURE 12-10 Management of equipment that will penetrate soft tissue or tooth structure.

• If the item is disposable, one should dispose of it properly after use on one patient.

• If the item is not disposable, one should clean it, package it, and heat sterilize it.

One should use the following approach to decontamination if the item will be used in the patient’s mouth but will not penetrate soft tissue or tooth structure (Figure 12-11).

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FIGURE 12-11 Management of equipment that will be used in a patient’s mouth but will not penetrate soft tissue or tooth structure.

• If the item is disposable, one should dispose of it properly after use on one patient.

• If the item is reusable, one should cover the parts that may become contaminated with an impervious barrier to prevent contamination.

• If the item is not disposable, cannot be covered completely, and becomes contaminated, one should clean it, package it, and heat sterilize it.

• If the item cannot be heat sterilized, one should clean and submerge it in a liquid sterilant/high-level disinfectant.

• If the item is not disposable and cannot be covered, heat sterilized, or submerged in a liquid agent, do not use the item.

One should use the following approach for items that are not used in the patient’s mouth (Figure 12-12).

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FIGURE 12-12 Management of equipment that will not be used in a patient’s mouth but that may become contaminated by touching or by spatter.

• If an item will not become contaminated with patient materials, one should just keep it clean.

• If an item will become contaminated with patient materials, one should clean it and when possible heat sterilize it.

• If the item cannot be heat sterilized because of its size or composition, one should clean and disinfect it.

Management of High-tech Equipment

New technologies are developed continually that aid the dental profession in treating patients. These technologies must be compatible with infection control or their use will be limited, but some technologies may present special infection control challenges, such as computers, cameras, and digital x-ray sensors.

For computers, one should avoid their contamination by performing hand hygiene before use. If this does not occur, recent studies have shown that the keyboards can be successfully decontaminated by using disinfectant wipes such as those containing quats. Also the same study showed that no damage occurred to the keyboards after 300 disinfecting procedures. Care should be taken that disinfectant wipes used should not contain excess fluid that might that might enter the base of the keyboard.

For 35-mm, video, and digital cameras, one should try to avoid their contamination. They are not designed to be disinfected. One should cover them with plastic sheeting or operate them only with clean hands.

Computer-aided design/computer-aided manufacturing devices (CAD/CAMS) use close-up intraoral pictures to design and manufacture restorations. These devices use a small camera lens on the end of a wire that transmits pictures from the patient’s mouth to a TV-type monitor. One should check with the manufacturer for proper decontamination of the camera and wire. One model has an outer removable prismatic device that slides over the camera lens, so the prism rather than the camera lens becomes contaminated. The prism can be sterilized in a dry heat sterilizer but not in a steam sterilizer, for the moist heat can damage the prism.

Digital radiography equipment needs special attention. This equipment uses intraoral sensors to provide a digital image that can be manipulated, viewed on a monitor, printed, or stored electronically. Because the sensors are reusable, one must handle them properly to prevent cross-contamination during subsequent use. One type of sensor, the charge-coupled device (CCD), is attached to a wire and should not be heat sterilized. Instead, one should cover the CCD with a plastic barrier that extends down the cable to prevent any contact with patient materials or contaminated hands. If the CCD does become contaminated, one may disinfect it by the spray-wipe-spray technique according to the manufacturer’s directions. Another type of digital x-ray sensor is the complementary metal-oxide semiconductor with active pixel sensors. This device is wired and should be treated with plastic barriers just like the CCD sensor. A third type of sensor, the photostimulable phosphor plate sensor, is wireless and is placed in the patient’s mouth much like a regular film packet. One must cover the device with a plastic barrier; it cannot become contaminated because it cannot be heat sterilized or chemically disinfected.

ASEPTIC DISTRIBUTION OF DENTAL SUPPLIES

Numerous supplies are used for patient care, and their storage and distribution present a major challenge to infection control. Examples of such items include cotton balls and rolls, gauze pads, floss, articulating paper, retraction cord, orthodontic wire, and tubes or bottles of dental materials, to mention only a few. The major problems relate to surface asepsis and how these supplies are obtained for use at chairside without cross-contamination.

Aseptic Retrieval

If one stores supply items in bulk, such as a container of cotton rolls, one must use an aseptic retrieval system (rather than saliva-coated gloved fingers) to avoid contamination of unused items in the container. Providing sterile forceps (to retrieve the supply item) with the instruments needed for each patient is one approach to this problem (Figure 12-13). Storing supplies (or instruments) in drawers at chairside lends itself to cross-contamination of the drawer handle (if not covered or precleaned and disinfected) or of bulk items inside (if aseptic retrieval is not used).

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FIGURE 12-13 Aseptic retrieval using sterilized cotton pliers issued for each patient.

Unit Dosing

The dental professional may protect supply containers, bottles, and tubes of materials used at chairside on more than one patient (multidose containers) with a surface cover for each patient or in some instances may preclean and disinfect these between patients. Many types of disposable supplies can be unit dosed, which means that the supplies are distributed or packaged in small numbers sufficient for care of just one patient and are placed at chairside before care begins. For example, a package may contain four cotton rolls, three cotton balls, two gauze pads, articulating paper, or whatever one anticipates for a single patient. Whatever is not used with a patient is discarded. Some supply items are unit dosed by the manufacturers, saving office staff time. Although unit dosing can solve some cross-contamination problems, unfortunately it can be expensive and wasteful if not organized properly.

SELECTED READINGS

Centers for Disease Control and Prevention. Guideline for infection control in dental health-care settings, 2003. MMWR. 2003;52(No. RR-17):1–68.

Miller, C.H. High-tech equipment: keeping it germ-free. Dent Prod Rpt. 2003;37:68–71.

Miller, C.H. Infection control. Dent Clin North Am. 1996;40:437–456.

Miller, C.H. High-tech equipment: keeping it germ-free. Dent Prod Rpt. 2003;37:68–71.

Miller, C.H. Labels, a must read. Dent Prod Rpt. 2006;40:82–86.

Miller, C.H., Strategies for infection control in dentistry. ADA guide to Dental Therapeutics, ed 3, Chicago, ADA Publishing, 2003. 551–566

Miller, C.H., Palenik, C.J. Sterilization, disinfection and asepsis in dentistry. In: Block S.S., ed. Sterilization, disinfection and preservation. ed 5. Philadelphia: Lea & Febiger; 2001:1049–1068.

Molinari, J.A., et al. Cleaning and disinfectant properties of dental surface disinfectants. J Am Dent Assoc. 1988;117:179–182.

Rutala, W.A., White, M.S., Gergen, M.F., Weber, D.J. Bacterial contamination of keyboards: Efficacy and functional impact of disinfectants. Inf Cont Hosp Epidemol. 2006;27:372–377.

Williams, H.N., Singh, R., Romberg, E. Surface contamination in the dental operatory. J Am Dent Assoc. 2003;134:325–330.

Review Questions

MULTIPLE CHOICE

______1. Which of the following microbes are not killed by intermediate-level disinfectants?

a. bacterial spores

b. tuberculosis agent

c. both a and b

d. neither a nor b

______2. Which of the following types of antimicrobial agents should be used on floors?

a. sterilant/high-level disinfectant

b. intermediate-level disinfectant

c. low-level disinfectant

______3. Which of the following antimicrobial agents should not be used for surface disinfection in dentistry?

a. sterilant/high-level disinfectant

b. intermediate-level disinfectant

c. low-level disinfectant

______4. Which of the following agents is not tuberculocidal?

a. iodophor

b. quaternary ammonium compound

c. phenolic

d. glutaraldehyde

______5. What types of surfaces should be covered rather than disinfected?

a. electric switches

b. surfaces that are difficult to clean

c. smooth surfaces

d. a and b

e. a and c

______6. Why should disinfectants used on clinical contact surfaces in dentistry contaminated with blood or saliva have tuberculocidal activity?

a. Tuberculosis is a common occupational disease of dental personnel, so a tuberculocidal agent is needed to prevent this type of spread in the office.

b. The tuberculosis agent is spread most commonly by touching contaminated surfaces.

c. Because the tuberculosis agent is one of the more resistant microbes to kill, tuberculocidal activity indicates sufficient potency to kill most other vegetative microbes.

______7. Liquid sterilants such as a glutaraldehyde should be used only on reusable items that can be submerged and are:

a. contaminated with blood

b. made of metal

c. heat sensitive

d. classified as critical (penetrate tissue)

______8. One should not use ______________ as a surface cover.

a. paper

b. plastic-backed patient napkin

c. plastic sheet

d. formed plastic bags

______9. Use of a disinfectant-type cleaner to preclean a contaminated operatory surface:

a. should not be done

b. starts the microbial killing process and helps protect the person doing the cleaning

c. eliminates the need to follow up with a disinfectant wipe

d. is necessary every time a surface cover is removed

______10. When can a liquid sterilant/high-level disinfectant achieve sterilization?

a. Sterilization will occur only when the solution is used at temperatures above 121° C.

b. Sterilization will occur when the solution is used only for the longer exposure times.